Enzyme Histochemistry - Gossrau, R.

. .

Sommaire:
A. Introduction.- B. General Considerations.- I. Conditions for the Histochemical Demonstration of Enzymes.- II. Terminology and Classification of Enzymes.- III. Principles of Reactions in Histochemical Methods for the Detection of Enzymes.- 1. Precipitation Reactions.- a. Precipitation Reactions with Metallic Cations.- b. Simultaneous Azo-Coupling.- c. Indigogenic Methods.- d. Tetrazolium Methods.- 2. Successive Azo-Coupling.- 3. Synthesizing Reactions.- 4. Substrate Film Procedures.- IV. Artifacts and Control Reactions.- V. Correlation of Histochemical and Biochemical Findings.- C. Preparation of Tissues.- I. Tissue Sampling.- II. Further Treatment.- 1. Fresh Tissue.- a. Freezing.- b. Preparations of Sections.- c. Freeze-drying of Cryostat Sections.- d. Freeze-Substitution.- 2. Fixation.- 3. Further Treatment of Fixed Tissue.- a. Washing.- b. Freezing and Preparation of Sections.- c. Embedding.- 4. Summary of Pretreatment of Tissue Specimens.- III. Incubation.- D. Detection Methods.- I. Instructions.- II. Hydrolases.- 1. Phosphatases.- a. Alkaline Phosphatase.- b. Acid Phosphatase.- c. 5?-Nucleotidase.- d. Glucose-6-Phosphatase.- e. Adenosine Triphosphatase.- f. Thiamine Pyrophosphatase.- g. Nucleoside Diphosphatase.- 2. Carboxylester-Hydrolases.- a. Nonspecific Esterases.- b. Lipase.- c. Cholinesterases.- 3. Glycosidases.- a. Acid ?-Glucosidase.- b. ?-Galactosidase.- c. Acid ?-Galactosidase.- d. ?-Mannosidase.- e. ?-N-Acetylglucosaminidase.- f. ?-GIucuronidase.- g. Disaccharidases.- ?. Lactase.- ?. Maltase.- ?. Sucrase.- ?. Trehalase.- 4. Peptidases.- a. Aminopeptidase M.- b. Dipeptidyl(amino)peptidase IV.- c. Dipeptidyl(amino)peptidase II.- d. Enteropeptidase.- e. ?-Glutamyltransferase.- 5. Sulfatases.- III. Transferases.- 1. Glycogen Phosphorylase.- 2. Glycogen (Starch) Synthase.- IV. Lyases.- 1. Fructose-Bisphosphate Aldolase.- 2. Carbonate Dehydratase.- V. Oxidoreductases.- 1. Oxidases.- a. Cytochrome c Oxidase.- b. Monophenol Monooxygenase.- c. Amine Oxidase.- d. D-Amino Acid Oxidase and Lactate-2-Monooxygenase.- e. Peroxidase.- 2. Dehydrogenases.- A. Coenzyme-lndependent Dehydrogenases.- a. Succinate Dehydrogenase.- b. Glycerol-3-Phosphate Dehydrogenase.- B. Tetrazolium Reductases.- C. Coenzyme-Dependent Dehydrogenases.- a. 3-Hydroxybutyrate Dehydrogenase.- b. UDPG Dehydrogenase.- c. Hydroxysteroid Dehydrogenases.- d. Glycerol-3-Phosphate Dehydrogenase (NAD+).- e. Glyceraldehydephosphate Dehydrogenase.- f. Glutamate Dehydrogenase.- g. Isoeitrate Dehydrogenase.- h. Glucose-6-Phosphate Dehydrogenase.- i. Phosphogluconate Dehydrogenase.- k. Lactate Dehydrogenase.- I. Malate Dehydrogenase.- m. Malate Dehydrogenase (NADP+).- E. Solutions and Buffers.- F. List of Firms.- G. References.- H. Subject Index and Source List.