Th9 Cells - Ritobrata Goswami

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Résumé :
This volume presents comprehensive laboratory protocols that have been used to generate Th9 cells, both in vitro and in vivo. The techniques described in Th9 Cells: Methods and Protocols study the role of Th9 cells in different inflammatory disease models, including allergic inflammation model, parasite model, tumor model, and EAE and IBD model. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and comprehensive, Th9 Cells: Methods and Protocols is a valuable resource for scientists in this field and will provide them with techniques to generate Th9 cells for specific downstream events.

Sommaire:

Th9 Cells: New Member of T Helper Cell Family.- IL-9: Function, Sources, and Detection.- IL-9 Signaling Pathway: An Update.- A Method to In Vitro Differentiate Th9 Cells from Mouse Na?ve CD4+ T Cells.- T Cell Receptor and Co-Stimulatory Signals for Th9 Generation.- Polarizing Cytokines for Human Th9 Cell Differentiation.- Determining the Frequencies of Th9 Cells from Whole Blood.- IL-9 Production by Nonconventional T Helper Cells.- Prediction and Validation of Transcription Factors Binding Sites in the IL-9 Locus.- Flow Cytometric Assessment of STAT Molecules in Th9 Cells.- Transcription Factors Downstream of IL-4 and TGF- ? Signals: Analysis by Quantitative PCR, Western Blot, and Flow Cytometry.- Retroviral Transduction and Reporter Assay: Transcription Factor Co-Operation in Th9 Cell Development.- Transcription Factor Binding Studies in CD4+ T Cells: siRNA Transfection, Chromatin Immunoprecipitation, and Liquid-Luminescent DNA-Precipitation Assay.- Defining Epigenetic Regulation of the Interleukin-9 Gene by Chromatin Immunoprecipitation.- Allergic Inflammation and Atopic Disease: Role of Th9 Cells.- Characterization of T Helper 9 (Th9) Cells in the Development of EAE and IBD.- B16 Lung Melanoma Model to Study the Role of Th9 Cells in Cancer.- Th9 Cells and Parasitic Inflammation: Use of Nippostrongylus and Schistosoma Models.- Isolation and Purification of Th9 Cells for the Study of Inflammatory Diseases in Research and Clinical Settings.