Microbial Toxins -

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Résumé :
This second edition volume expands on the previous editions with detailed protocols on (cyano) bacterial and mold fungus toxins, and a specific focus on Gram-negative endotoxins (lipopolysaccharides). The chapters in Microbial Toxins: Methods and Protocols, Second Edition present techniques that cover topics such as detection of cholera toxin by an immunochromatographic test strip; label-free immune-sensors for the fast detection of Listeria in food; endotoxin removal from Escherichia coli bacterial lysate using biphasic liquid system; and micromethods for isolation and structural characterization of lipid A, and polysaccharide regions of bacterial lipopolysaccharides. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Microbial Toxins: Methods and Protocols, Second Edition is a valuable and useful resource for scientists who are interested in this field, and is a great tool for researchers who are looking to learn about new, particular techniques to further enhance their work.

Sommaire:

Detection of Cholera Toxin by an Immunochromatographic Test Strip.- Electrochemical Aptamer Scaffold Biosensors for Detection of Botulism and Ricin Proteins.- A Cell-Based Fluorescent Assay to Detect the Activity of AB Toxins that Inhibit Protein Synthesis.- Molecular Methods for Identification of Clostridium tetani by Targeting Neurotoxin.- Label-Free Immuno-Sensors for the Fast Detection of Listeria in Food.- Aptamer-Based Trapping: Enrichment of Bacillus cereus Spores for Real-Time PCR Detection.- Detection of Yersinia pestis in Complex Matrices by Intact Cell Immunocapture and Targeted Mass Spectrometry.- A Method to Prepare Magnetic Nanosilicate Platelets for Effective Removal of Microcystis aeruginosa and Microcystin LR.- An Immunochromatographic Test Strip to Detect Ochratoxin A and Zearalenone Simultaneously.- Endotoxin Removal from Escherichia coli Bacterial Lysate using Biphasic Liquid System.- Fourier Transform Infrared Spectroscopy as a Tool in Analysis of Proteus mirabilis Endotoxins.- Laser Interferometry Method as a Novel Tool in Endotoxin Research.- Endotoxin Entrapment on Glass via C18 Self-Assembled Monolayers and Rapid Detection using Drug-Nanoparticle Bioconjugate Probes.- A Bioassay for Determination of Lipopolysaccharides and Lipoproteins.- Capillary Electrophoresis Chips for Fingerprinting Endotoxin Chemotypes and Subclasses.- Micromethods for Isolation and Structural Characterization of Lipid A, and Polysaccharide Regions of Bacterial Lipopolysaccharides.- Mass Spectrometry for Profiling LOS and Lipid A Structures from Whole-Cell Lysates: Directly from a Few Bacterial Colonies or from Liquid Broth Cultures.