Modern Clinical Molecular Techniques -

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Résumé :
This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today?s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure.? It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Sommaire:
Preface Acknowledgement Contributors ? Section I?? Current Molecular Laboratory Methods 1??????? Specimen Collection, Handling and Processing Lindsy Hengesbach and John Gerlach 2??????? DNA/RNA Isolation and Quantitation Jing Wang 3??????? Test Validation Cindy McCloskey and S Terence Dunn 4??????? In Vitro Amplification Methods in Molecular Diagnostics C. Alexander Valencia and Bradford Coffee 5??????? Whole Genome Sequencing in the Clinical Laboratory Tina Hambuch, Brad Sickler, Arnold Liao, Suneer Jain, and Philip D. Cotter 6??????? Estimation of Exon Dosage Using Real-Time Quantitative Polymerase Chain Reaction Juliana Purrazzella and Arupa Ganguly 7??????? Reporting Clinical Molecular Genetic Laboratory Results Victor Wei Zhang and Lora J. H. Bean Section II Clinical Molecular Infectious Disease Testing 8??????? HIV-1 Genotyping and Virtual Phenotyping Fangling Xu and Jianli Dong 9??????? COBAS? Ampliprep/Taqman HIV-1 Test Tavat A. Buraruk and Charles E. Stager 10??? Hepatitis C Virus (HCV) Genotype Assay (LiPA) Charles E. Stager and Tavat A. Buraruk 11??? COBAS? Ampliprep/Taqman HCV Test 12??? Epstein-Barr Virus and Cytomegalovirus Viral Load Monitoring by Quantitative Real-Time Polymerase Chain Reaction Su S. Chen, Seema Hai, and Rajyalakshmi Luthra 13??? Quantification of BK Viral Load Hai Wu and Jianli Dong 14??? Molecular Diagnosis and Epidemiology of Rabies Rodney E. Rohde and Bonny C. Mayes 15??? Pyrosequencing for Bacterial Identification Laura Millecker and Ruth Ann Luna 16??? Other Pathogens of Significant Public Health Concern Chris L. McGowin, Rodney E. Rohde, and Greg C. Whitlock Section III Clinical Molecular Oncology Testing 17??? Gene Rearrangements Testing Siby Sebastian, Juan Du, and Rizwan Naeem 18??? Mutation Analysis in Cancer Keyur Pravinchandra Patel, John Galbincea, ?and Rajyalakshmi Luthra 19??? Minimal Residual Disease Wesley Greaves and Rajyalakshmi Luthra 20??? Pyrosequencing in Cancer Bedia A. Barkoh, Rajyalakshmi Luthra, and Shalini Verma 21??? Clinical Applications of Microarrays in Cancer Marilyn M. Li, Ankita Patel, and Xiaofeng Hu 22??? Basic Theories and Clinical Applications of Molecular Flow Cytometry Hua Kang Wu, Hongli Chen, and Peter Hu Section IV Clinical Molecular Pre/Postnatal Testing 23??? Molecular Prenatal Diagnostic Testing Joanna Wiszniewska and Christine M. Eng 24??? PCR Based Diagnosis of Fragile X Syndrome? Daniel J. Dykas and Allen E. Bale Section V Human Identity Testing 25??? Microsatellite Testing Short Tandem Repeat Profile Generation Robin DeVille Guidry and Laura Gahn 26??? Parentage Laura Gahn and Robin DeVille Guidry Section VI Clinical Molecular Training 27??? Diagnostic Molecular Training Peter Hu, Patrick A. Lennon, and Madhuri Hegde 28??? Molecular Diagnostics Point of Care Testing Training Chang W. Lee, and Peter Hu