Biomass Conversion -

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Résumé :
Biomass conversion research is a combination of basic science, applied science, and engineering testing and analysis.? Conversion science includes the initial treatment (called pre-treatment) of the feedstock to render it more amenable to enzyme action, enzymatic saccharification, and finally product formation by microbiological or chemical processes. ?In Biomass Conversion: Methods and Protocols, expert researchers in the field detail methods which are now commonly used to study biomass conversion. These methods include Biomass Feedstocks and Cellulose, Plant Cell Wall Degrading Enzymes and Microorganisms, and Lignins and Hemicelluloses. Written in the highly successful Methods in Molecular Biology? series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting informed, reproducible results in the laboratory.

Sommaire:
1. Challenges for Assessing the Performance of Biomass Degrading Biocatalysts Michael E. Himmel, Stephen R. Decker, and David K. JohnsonPart I: Biomass Feedstock and Cellulose?2. Overview of Computer Modeling of CelluloseMalin Bergenstr?hle-Wohlert,?and John W. Brady3. Imaging Cellulose Using Atomic Force MicroscopyShi-You Ding and Yu-San Liu4. Preservation and Preparation of Lignocellulosic Biomass Samples for Multi-scale Microscopy AnalysisBryon S. Donohoe, Peter N. Ciesielski, and Todd B. Vinzant?5. Coherent Raman Microscopy Analysis of Plant Cell WallsYining Zeng, Michael E. Himmel, and Shi-You Ding6. Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling Sivakumar Pattathil, Utku Avci, Jeffrey S. Miller, and Michael G. Hahn?7. Immunological Approaches To Plant Cell Wall and Biomass Characterization:? Immunolocalization of Glycan EpitopesUtku Avci, Sivakumar Pattathil, and Michael G. Hahn8. A Method to Evaluate Biomass Accessibility in Wet State Based on ThermoporometryBon-Wook Koo and Sunkyu ParkPart II: Plant Cell Wall9. Cellulase ProcessivityDavid B. Wilson and Maxim Kostylev?10. A Simple Method for Determining Specificity of Carbohydrate-Binding Modules for Purified and Crude Insoluble Polysaccharide SubstratesOren Yaniv, Sadanari Jindou, Felix Frolow, Raphael Lamed, and Edward A. Bayer11. Bacterial Cadherin Domains as Carbohydrate Binding Modules: Determination of Affinity Constants to Insoluble Complex PolysaccharidesMilana Fraiberg, Ilya Borovok, Ronald M. Weiner, Raphael Lamed, and Edward A. Bayer?12. Affinity Electrophoresis as a Method for Determining Substrate-Binding Specificity of Carbohydrate-Active Enzymes for Soluble PolysaccharidesSarah Mora?s, Raphael Lamed, and Edward A. Bayer13. Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence MicroscopyYu-San Liu, Shi-You Ding, and Michael E. Himmel??14. Bioprospecting Metagenomics for New Glycoside HydrolasesJack Gilbert, Luen-Luen Li, Safiyh Taghavi, Sean M. McCorkle, Susannah Tringe, and Daniel van der Lelie15. Anaerobic High-Throughput Cultivation Method for Isolation of Thermophiles Using Biomass-Derived SubstratesScott D. Hamilton-Brehm, Tatiana A. Vishnivetskaya, Steve Allman, Jonathan Mielenz, and James G. Elkins?16. Assessing the Protein Concentration in Commercial Enzyme Preparations? William S. Adney, Nancy Dowe, Edward W. Jennings, Ali Mohagheghi,John Yarbrough, and James D. McMillan17.? Reducing the Effect of Variable Starch Levels in Biomass Recalcitrance Screening Stephen R. Decker, Melissa Carlile, Michael J. Selig, Crissa Doeppke, Mark Davis, Robert Sykes, Geoffrey Turner, and Angela Ziebell?18. Analysis of Transgenic Glycoside Hydrolases Expressed in Plants: T. reesei CBH I and A. cellulolyticus EIRoman Brunecky, John O. Baker , Hui Wei, Larry E. Taylor, Michael E. Himmel, and Stephen R. DeckerPart III: Lignins and HEmicelluloses 19. Structural Characterization of the Heteroxylans from Poplar and SwitchgrassKoushik Mazumder, Maria J. Pe?a, Malcolm A. O'Neill, William S. York?20. Laser Microdissection and Genetic Manipulation Technologies to Probe Lignin Heterogeneity and Configuration in Plant Cell WallsOliver R. A. Corea, Chanyoung Ki, Claudia L. Cardenas, Laurence B. Davin, and Norman G. Lewis?21. Lignin-Degrading Enzyme ActivitiesYi-ru Chen, Simo Sarkanen and Yun-Yan Wang?