Ras Signaling -

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Résumé :

The Ras Superfamily of Small GTPases: The Unlocked Secrets.- A Novel Method for the Production of Fully-Modified K-Ras 4B.- Metabolic Labeling of Ras with Tritiated Palmitate to Monitor Palmitoylation and Depalmitoylation.- Ubiquitination of Rac1 by Inhibitors of Apoptosis (IAPs).- Ras GTPases are Both Regulators and Effectors of Redox Agents.- Biophysical and Proteomic Characterization Strategies for Cysteine Modifications in Ras GTPases.- Use of the Yeast Two-Hybrid Technology to Isolate Molecular Interactions of Ras GTPases.- Screening for MAPK Modulators Using an In-Cell Western Assay.- Behavioral Methods for the Study of the Ras-ERK Pathway in Memory Formation and Consolidation: Passive Avoidance and Novel Object Recognition Tests.- Functional Phosphoproteomics of Oncogenic KRAS Signaling.- Pull-Down Assay for Analysis of Integrin-Mediated Activation of Rap Proteins in Adherent Platelets.- Combined Pulldown and Time-Lapse Microscopy Studies for Determining the Role of Rap1 in the Crosstalk Between Integrins and Cadherins.- Fluorescence Microscopy Study of Rap1 Subcellular Localization.- An?In Vitro?System to Evaluate the Scaffold Function of the RalA Effector Protein RalBP1.- Analysis of the Rit Subfamily GTPase-Mediated Signaling and Neuronal Differentiation and Survival.- Immunofluorescence Methods in Studies of the GTPase RAN and Its Effectors in Interphase and in Mitotic Cells.- High Resolution Scanning Electron Microscopy for the Imaging of Nuclear Pore Complexes and Ran-Mediated Transport.- Effector Recruitment Method to Study Spatially Regulated Activation of Ras and Rho GTPases.- Real-Time Visualization and Quantification of Native Ras Activation in Single Living Cells.- Nanoclustering and Heterogeneous Membrane Diffusion of Ras Studied by FRAP and RICS Analysis.- Analyzing the Roles of Rho GTPases in Cancer Cell Migration with a Live Cell Imaging 3D-Morphology-Based Assay.- Analysis of Rho-GTPase-Induced Localization of Nanoscale AdhesionsUsing Fluorescence Nanoscopy.- Yeast as a Model for Ras Signaling.- Methods to Study the Ras2 Protein Activation State and the Subcellular Localization of Ras-GTP in Saccharomycescerevisiae.-RAS? Proteins Signaling in the Early Metazoan Dictyostelium discoideum.