Molecular Fluorescence - Bernard Valeur

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Résumé :
Molecular Fluorescence

This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments.

The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips.

For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology....

Biographie:

INTRODUCTION

What Is Luminescence?

A Brief History of Fluorescence and Phosphorescence

Photoluminescence of Organic and Inorganic Species: Fluorescence or Phosphorescence?

Various De-Excitation Processes of Excited Molecules

Fluorescent Probes, Indicators, Labels, and Tracers

Ultimate Temporal and Spatial Resolution: Femtoseconds, Femtoliters, Femtomoles, and Single-Molecule Detection

PART I: PRINCIPLES

ABSORPTION OF ULTRAVIOLET, VISIBLE, AND NEAR-INFRARED RADIATION

Electronic Transitions

Transition Probabilities: The Beer - Lambert Law, Oscillator Strength

Selection Rules

The Franck -Condon Principle

Multiphoton Absorption and Harmonic Generation

CHARACTERISTICS OF FLUORESCENCE EMISSION

Radiative and Nonradiative Transitions between Electronic States

Lifetimes and Quantum Yields

Emission and Excitation Spectra

STRUCTURAL EFFECTS ON FLUORESCENCE EMISSION

Effects of the Molecular Structure of Organic Molecules on Their Fluorescence

Fluorescence of Conjugated Polymers (CPs)

Luminescence of Carbon Nanostructures: Fullerenes, Nanotubes, and Carbon Dots

Luminescence of Metal Compounds, Metal Complexes, and Metal Clusters

Luminescence of Semiconductor Nanocrystals (Quantum Dots and Quantum Rods)

ENVIRONMENTAL EFFECTS ON FLUORESCENCE EMISSION

Homogeneous and Inhomogeneous Band Broadening - Red-Edge Effects

General Considerations on Solvent Effects

Solvent Relaxation Subsequent to Photoinduced Charge Transfer (PCT)

Theory of Solvatochromic Shifts

Effects of Specific Interactions

Empirical Scales of Solvent Polarity

Viscosity Effects

Fluorescence in Gas Phase: Supersonic Jets

EFFECTS OF INTERMOLECULAR PHOTOPHYSICAL PROCESSES ON FLUORESCENCE

EMISSION

Introduction

Overview of the Intermolecular De-Excitation Processes of Excited Molecules Leading to Fluorescence Quenching

Photoinduced Electron Transfer

Formation of Excimers and Exciplexes

Photoinduced Proton Transfer

FLUORESCENCE POLARIZATION: EMISSION ANISOTROPY

Polarized Light and Photoselection of Absorbing Molecules

Characterization of the Polarization State of Fluorescence (Polarization Ratio and Emission Anisotropy)

Instantaneous and Steady-State Anisotropy

Additivity Law of Anisotropy

Relation between Emission Anisotropy and Angular Distribution of the Emission Transition Moments

Case of Motionless Molecules with Random Orientation

Effect of Rotational Motion

Applications

EXCITATION ENERGY TRANSFER

Introduction

Distinction between Radiative and Nonradiative Transfer

Radiative Energy Transfer

Nonradiative Energy Transfer

Determination of Distances at a Supramolecular Level Using FRET

FRET in Ensembles of Donors and Acceptors

FRET between Like Molecules: Excitation Energy Migration in Assemblies of Chromophores

Overview of Qualitative and Quantitative Applications of FRET

PART II: TECHNIQUES

STEADY-STATE SPECTROFL UOROMETRY

Operating Principles of a Spectrofl uorometer

Correction of Excitation Spectra

Correction of Emission Spectra

Measurement of Fluorescence Quantum Yields

Possible Artifacts in Spectrofl uorometry

Measurement of Steady-State Emission Anisotropy: Polarization Spectra

TIME-RESOLVED FLUORESCENCE TECHNIQUES

Basic Equations of Pulse and Phase-Modulation Fluorimetries

Pulse Fluorimetry

Phase-Modulation Fluorimetry

Artifacts in Time-Resolved Fluorimetry

Data Analysis

Lifetime St...

Sommaire:

Bernard Valeur received his engineering diploma from the ?cole Sup?rieure de Physique et de Chimie Industrielles de Paris (E.S.P.C.I.) and his PhD degree from the Universit? Pierre-et-Marie-Curie (Paris, France), followed by postdoctoral research at the University of Illinois at Urbana-Champaign (USA). After being an associate professor at E.S.P.C.I, he became full professor of physical chemistry at the Conservatoire National des Arts et M?tiers (Paris) in 1979, where he is emeritus professor since 2008. Professor Valeur is a member of the laboratory Photophysique et Photochimie Supramol?culaires et Macromol?culaires at the ?cole Normale Sup?rieure de Cachan since 1996. From 1995 to 2000 he served as an elected member of the French Comit? National de la Recherche Scientifique. He is the winner of the 2012 Gregorio Weber Award for Excellence in Fluorescence Theory and Applications and author of over 170 articles or book chapters, five books, and the editor of one book. In addition, he is a member of several editorial boards.

M?rio Nuno Berberan-Santos graduated in chemical engineering from Instituto Superior T?cnico (IST, Technical University of Lisbon, Portugal). After a brief stay at the National Research Council of Canada (Ottawa), he received his PhD in chemistry from IST in 1989. He was a post-doctoral fellow with Bernard Valeur at Conservatoire National des Arts et M?tiers (Paris, France), and at Laboratoire pour l'Utilisation du Rayonnement Electromagn?tique (Univ. Paris-Sud, Or- say, France). He is full professor of Physical Chemistry at IST, and was invited full professor at the ?cole Normale Sup?rieure de Cachan (France). He is a member of several editorial advisory boards and is president of the Portuguese Chemical Society (2010-2012). He has authored over 180 publications, including 150 papers in scientific journals, several book chapters, and was the editor of one book.