The Complement System -

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Résumé :

Overview.- Evaluation of Complement Function by ELISA.- Classical Complement Pathway Component C1q - Purification of Human C1q, Isolation of C1q Collagen-like and Globular Head Fragments and Production of Recombinant C1q - Derivatives. Functional Characterization.- Classical Complement Pathway Components C1r and C1s - Purification from Human Serum and in Recombinant Form and Functional Characterization.- Expression and Purification Methods for the Production of Recombinant Human Complement Component C2.- Purification and Characterisation of human and Mouse Complement C3.- Purification of Human Complement Protein C5.- Quantification of Complement C5b-9 Binding to Cells by Flow Cytometry.-Challenges and Opportunities in Fractionation of Recombinant Human Mannan-binding Lectin.- Genotyping of FCN and MBL2 Polymorphisms using Pyrosequencing.- Assay for Estimation of the Functional Activity of the Mannan Binding Lectin Pathway of the Complement System.- Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins.- Purification and Functional Analysis of Human Properdin.- Purification and Functional Characterization of C4b-binding Protein (C4BP).- Purification and Functional Characterization of Factor I.- C1 inhibitor - Quantification and Purification.- Purification, Quantification and Functional Analysis of Complement Factor H.- Factor H-related Proteins.- Functional Evaluation of Factor H genetic and Acquired Abnormalities...

Biographie:
Application for Atypical Hemolytic Uremic Syndrome (aHUS).- Anti-Factor H Auto Antibodies Assay.- Purification of Native Surfactant Protein SP-A from Pooled Amniotic Fluid and Bronchoalveolar Lavage.- Purification of Surfactant Protein D (SP- D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage.- Functional Analysis of C5a Effector Responses In Vitro and In Vivo.- Detection of Complement Receptors 1 and 2 on Mouse Splenic B Cells using Flow Cytometry.- Quantificationof Complement Receptor 2 Calcium Signaling Enhancement Using Flow Cytometry.- Purification of C1q Receptors and Functional Analysis.- Detection of Cell Membrane-bound CD46 using Flow Cytometry.- Detection of CD59 using Flow Cytometry.- Functional Analysis of CD59 using Complement Dependent Cytotoxicity Assay.- Detection of Soluble CR3 (CD11b/CD18) by Time-resolved Immunofluorometry.- Analysis of the Complement Activation in Mice.- Opsonophagocytic Assay.