The Myc Gene -

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Résumé :
Myc controls multiple cellular functions, including cell proliferation, growth, differentiation and death, both directly and indirectly, through its modulation of downstream transcriptional programs. In The Myc Gene: Methods and Protocols, experts in the field summarize the standard and novel techniques that allow the studying of Myc mechanism of action in normal and cancer cells, in vitro and in vivo, in one succinct manual. Written in the highly successful Methods in Molecular Biology ?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Sommaire:
1. The Myc World Within ReachNicole M. Sodir and Laura Soucek?2. Methods for the Expression, Purification, Preparation and Biophysical Characterization of Constructs of the c-Myc and Max b-HLH-LZsMarie-Eve Beaulieu, Fran?ois-Olivier Mc Duff, Mika?l B?dard, Martin Montagne and Pierre Lavigne?3. Analyzing Myc in Cell Transformation and EvolutionMarkus Hartl and Klaus Bister?4. Identifying Myc InteractorsRomina Ponzielli, William B. Tu, Igor Jurisica, ?and Linda Z. Penn?5. Detection of c-Myc Protein-Protein Interactions And Phosphorylation Status by ImmunoprecipitationColin J. Daniel, Xiaoli Zhang and Rosalie C. Sears?6. An Efficient Way of Studying Protein-Protein Interactions Involving HIF-a, c-Myc, and Sp1Kenneth K.-W. To and L. Eric Huang?7. Methods for Determining Myc-induced ApoptosisDan Lu and Trevor D. Littlewood?8. Methods to Study Myc-regulated Cellular SenescenceVedrana Tabor, Matteo Bocci and Lars-Gunnar Larsson?9. Chromatin Immunoprecipitation Assays for Myc and N-MycBonnie L. Barrilleaux, Rebecca Cotterman, and Paul S. Knoepfler?10. Methods to Quantify microRNAs in the Myc Gene Network for Posttranscriptional Gene RepressionRui Song, Nicole Sponer, and Lin He?11. Genome-wide Analysis of c-MYC-regulated mRNAs and miRNAs, and c-MYC DNA-binding by Next Generation SequencingRene Jackstadt, Antje Menssen and Heiko Hermeking?12. A High Throughput siRNA Screening Platform to Identify MYC Synthetic Lethal Genes as Candidate Therapeutic TargetsCarla Grandori?13. Investigating Myc-dependent Translational Regulation in Normal and Cancer CellsJohn T. Cunningham, Michael Pourdehnad, Craig R. Stumpf, and Davide Ruggero?14. Studying Myc's Role in Metabolism Regulation Anne Le and Chi V. Dang?15. Generation of a Tetracycline Regulated Mouse Model of MYC-induced T-cell Acute Lymphoblastic LeukemiaKavya Rakhra and Dean W. Felsher?16. Methods to Assess Myc Function in Intestinal Homeostasis, Regeneration and TumourigenesisDavid J Huels, Patrizia Cammareri, Rachel A Ridgway, Jan P Medema and Owen J Sansom?17. Detection of c-myc Amplification in Formalin-fixed Paraffin-Embedded Tumor Tissue by Chromogenic in situ Hybridization (CIHS)Natasa Todorovic-Rakovic ?18. Cell-Based Methods for the Identification of MYC-Inhibitory Small Molecules Catherine A. Burkhart, Michelle Haber, Murray D. Norris, Andrei V. Gudkov, and Mikhail A. Nikiforov