Mass Spectrometry Data Analysis in Proteomics -

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Résumé :
Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably.? Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics.? Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. ?Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics.

Biographie:
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Sommaire:

1. Introduction to Mass Spectrometry-Based Proteomics

??????????? Rune Matthiesen and Jakob Bunkenborg

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2. LC-MS Spectra Processing

??????????? Rune Matthiesen

3. Isotopic Distributions

??????????? Alan L. Rockwood and Magnus Palmblad

4. Retention Time Prediction and Protein Identification

??????????? Alex A. Henneman and Magnus Palmblad

5. Algorithms for Database Dependent Search of MSMS Data

6. Interpretation of Tandem Mass Spectra of Post-Translationally Modified Peptides

??????????? Jakob Bunkenborg and Rune Matthiesen

7. Improving Peptide Identification Using Empirical Scoring Systems

??????????? Robert J. Chalkley

8. Methods and Algorithms for Quantitative Proteomics by Mass Spectrometry

??????????? Rune Matthiesen and Ana Sofia Carvalho

9. Computational Approaches to Selected Reaction Monitoring (SRM) Assay Design

??????????? Conrad Bessant and Jun Fan

10. Feature Selection and Machine Learning with Mass Spectrometry Data

??????????? Susmita Datta

11. Considerations in the Analysis of Hydrogen Exchange Mass Spectrometry Data

??????????? Thomas E. Wales, Michael J. Eggertson, and John R. Engen

12. Permethylated N-Glycan Analysis with Mass Spectrometry

??????????? Zhenxin Lin and David M. Lubman

13. Mass Spectrometry Methods for Studying Glycosylation in Cancer

??????????? Hugo Os?rio and Celso A. Reis

14. Proteomics Data Exchange and Storage: The Need for Common Standards and Public Repositories

??????????? Rafael C. Jim?nez and Juan Antonio Vizca?no

15. Tools for Protein Post Translational Modifications Analysis: FAK, A Case Study

??????????? Catarina Fonseca, Paula Voabil, Ana Sofia Carvalho, and Rune Matthiesen

16. Proteomic Strategies to Characterize Signaling Pathways

??????????? H.C. Harsha, Sneha M. Pinto, and Akhilesh Pandey

17. Simple Proteomics Data Analysis in the Object-Oriented PowerShell

??????????? Yassene Mohammed and Magnus Palmblad