Tiling Arrays -

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Résumé :
?In Tiling Arrays: Methods and Protocols,? expert researchers in the field detail many of the methods which are now commonly used to study tiling microarrays in genomic discovery . These include methods and applications for transcriptional regulation, expression, genetic and epigenetic regulation, as well as techniques and skills on tiling microarray data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. ? Authoritative and practical, Tiling Arrays: Methods and Protocols ?seeks to provide scientists with a comprehensive and down to earth approach to maximize results.

Sommaire:
Part I: OverView1. A Brief Introduction to Tiling Microarrays: Principles, Concepts, and ApplicationsChristophe Lemetre and Zhengdong D. Zhang ??Part II: Transcriptional Regulation and Expression2. Design of Tiling Arrays and their Application to Bacterial Transcriptome AnalysisTakeshi Akama, Kazuaki Nakamura, Akito Tanoue, and Koichi Suzuki?3. Transcript Profiling in Arabidopsis with Genome Tiling MicroarraysDiana Coman, Wilhelm Gruissem, and Lars Hennig?4. Genome-wide Analysis of Transcription Factor Binding Sites in Skeletal Muscle Cells using ChIP-seqChung-Il An and Nobuko Hagiwara?5. Analysis of Allele-Specific Gene Expression Using a Target-Oriented Tiling Microarray AssayHuiyong Zhang, Xing-Wang Deng, and Lei Li??Part III: Genetics and Epigenetics Regulation6. Detection of Epigenetic Alterations Using Tiling ArraysHoi-Hung Cheung and Owen M Rennert?7. Investigating Gene Promoter Methylation in a Mouse Model of Status EpilepticusSuzanne F. C. Miller-Delaney, Sudipto Das, Raymond L. Stallings, and David C. Henshall?Part IV: Data Analysis8. Integrative Analysis of ChIP-chip and ChIP-seq DatasetLihua Julie Zhu?9. HAT: A Novel Statistical Approach to Discover Functional Regions in the GenomeErdogan Taskesen, Bas Wouters, and Ruud Delwel?10. Inference of Alternative Splicing from Tiling Array DataJohannes Eichner?11. Analysis of in vivo Occupancy of Aebp1, a Transcription Factor, using High Resolution Tiling ArraySwati Sinha, Sainitin Donakonda, ?and Satyanarayana MR Rao?12. Application of the Simple and Efficient MPeak Modeling in Binding Peak Identification in CHIP-CHIP StudiesMing Zheng, Yunmin Li, ?and Yun-Fai Chris Lau?13. Evaluation of MeDIP-chip in the Context of Whole Genome Bisulphite Sequencing (WGBS-seq) in ArabidopsisRen? Wardenaar, Haiyin Liu, Vincent Colot, Maria Colom?-Tatch?, Frank Johannes?14. Mapping Genomic Features of Tiling Microarray Data by TileMapperHoi-Hung Cheung, Janek Claus, Sumeeta Singh, Chandan Sastry, Owen M? Rennert, Wai-Yee Chan, Tin-Lap Lee?