Type 2 Diabetes -

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Résumé :
Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology? series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.

Sommaire:
1) Selecting the 'Right' Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research
Edward H. Leiter

2) Nutritional Models of Type 2 Diabetes Mellitus
Beverly Sara Muhlhausler

3) The Isolation and Purification of Rodent Pancreatic Islets of Langerhans
Jacqueline F. O'Dowd

4) The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans
Anna L. Nolan and Jacqueline F. O'Dowd

5) The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ?-Cell Lines
Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell

6) In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function
Anna L. Nolan

7) Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells
Dany Muller, Peter M. Jones, and Shanta J. Persaud

8) Laser Capture Microdissection of Human Pancreatic b -Cells and RNA Preparation for Gene Expression Profiling
Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, and Gordon C. Weir

9) In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells
Wan-chun Li

10) The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes
Nicky Konstantopoulos and Juan Carlos Molero-Navajas

11) Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell Size
Eduard Montanya and No?lia T?llez

12) Morphology of Pancreatic Islets: A Time Course of Pre-Diabetes in Zucker Fatty Rats
Petra Augstein and Eckhard Salzsieder

13) Fluorescent Immunohistochemistry and in situ Hybridization Analysis of Pancreas
Xiuli Wang, Shundi Ge, and Gay M. Crooks

14) The Measurement of Insulin Secretion Using Pancreas Perfusionin the Rodent
Edward T. Wargent

15) Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo
Jason K. Kim

16) Gene Expression Analysis in Diabetes Research
Peter White and Klaus H. Kaestner

17) Gene Expression Mining in Type 2 Diabetes Research
Donald R. Dunbar

From the reviews: This book provides in 17 chapters laboratory protocols used in diabetes research. It describes methods using new technologies in studying pancreatic beta cells. For research diabetologists. (Pediatric Endocrinology Reviews, Vol. 7 (4), June, 2010)