Diagnostic Techniques in Public Health -

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Résumé :

Comprehensive overview of the various diagnosis techniques employed in disease detection and public health crisis management

Diagnostic Techniques in Public Health: Analytical and Chemometric Approaches in Disease Detection and Management explores the roles of advanced chemometric approaches and modern diagnostic tools in addressing complex analytical challenges in global health. The book provides a systematic examination of major international public health emergencies, including COVID-19 and Monkeypox, as defined by the World Health Organization.

This volume brings together carefully curated chapters that integrate perspectives from molecular biology, analytical chemistry, nanotechnology, biomedical engineering, and informatics, addressing how diagnostic science can evolve to meet the demands of a rapidly changing and interconnected world.

Diagnostic Techniques in Public Health provides in-depth coverage of representative topics, including:

• Molecular diagnostic technologies, ranging from classical polymerase chain reaction to next-generation isothermal amplification methods
• ELISA-based immunoassays and genomic sequencing as key tools for pathogen surveillance
• Emerging technologies such as CRISPR-based diagnostics and microfluidic systems enabling high automation, multiplexing, and reagent efficiency
• Plasmonic biosensors and flexible bioelectronics, highlighting advances toward non-invasive, continuous, and personalized health monitoring

Bridging advanced analytical methodologies with public health applications, Diagnostic Techniques in Public Health serves as a valuable reference for analytical, medicinal, and pharmaceutical chemists, molecular biologists, and public health professionals....

Biographie:

Dr. Jinghong Li is an Academician at the Chinese Academy of Sciences and a Cheung Kong Professor in the Department of Chemistry at Tsinghua University, China. He is also the Director of the Academic Committee in the Department of Chemistry at Tsinghua University.

Dr. Yan Du is Professor at Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CAS, China). She has been working on biosensing and molecular diagnosis for more than 20 years....

Sommaire:

Preface xiii

1 Analytical and Chemometric Approaches in Public Health Emergencies: Global Significance, Challenges, and Disease Detection Strategies 1
Yan Du and Jinghong li

1.1 Beyond Infectious Threats: Expanding the Horizons of Global Health Challenges 2

1.1.1 Introduction to Global Health Challenges 2

1.1.2 Determinants Influencing the Spread and Management of Diseases 2

1.2 Tracing the Seven Public Health Emergencies of International Concern Announcements by the World Health Organization: From H1N1 to Monkeypox 3

1.2.1 Overview of the World Health Organization's Criteria for Declaring a Public Health Emergency of International Concern 3

1.2.2 List of Public Health Emergencies of International Concern Declarations with Dates and Brief Descriptions 3

1.2.2.1 H1N1 Influenza Pandemic (2009) 3

1.2.2.2 Poliovirus (2014) 4

1.2.2.3 Ebola Virus Disease Outbreak in West Africa (2014) 4

1.2.2.4 Zika Virus (2016) 4

1.2.2.5 Ebola Virus Disease Outbreak in the Democratic Republic of Congo (2019) 4

1.2.2.6 COVID-19 Pandemic (2020) 4

1.2.2.7 Monkeypox (2022) 5

1.2.3 Comparative Analysis of the Responses to Each Public Health Emergency of International Concern and the Evolution of Global Health Security Measures 5

1.3 A Close Look at Smallpox, Severe Acute Respiratory Syndrome, and Influenza Variations 5

1.3.1 Eradication Effort and Lessons Learned from Smallpox 6

1.3.2 Etiology, Transmission, and the Global Response of Severe Acute Respiratory Syndrome 6

1.3.3 Evolution of Influenza Strains and Their Implications for Global Pandemics 6

1.4 Analytical and Chemometric Approaches in Major Public Health Emergencies 6

1.4.1 Severe Acute Respiratory Syndrome Virus Antigen Immunoassay 7

1.4.2 Rapid Detection of COVID-19 and Its Variants 7

1.4.2.1 High-Sensitivity Rapid Detection of COVID- 19 7

1.4.2.2 Single-Base Resolution Identification and Rapid Screening of COVID- 19

Variants 8

1.4.2.3 Single-Base Resolution Nucleic Acid Rapid Detection Technology Assisting Vaccine Development and Drug Screening 9

1.4.3 Genetic Evolution Monitoring of the Monkeypox Virus 10

1.5 Conclusion and Discussion 12

References 13

2 Mastering Clinical Diagnostics: Modern Strategies for PHEIC Responses 19
XinxinShenandXuejunMa

2.1 Clinical Evaluation Comprehensive Pathway: From Cutting-edge Laboratory Analysis 19

2.2 Recombinase-aided Isothermal Amplification of Nucleic Acids: A New Dawn in Detection and Analysis 20

2.2.1 Introduction 20

2.2.2 Principle of the Recombinase-aided Amplification 21

2.2.3 The Detection of the RAA Products 22

2.2.4 Applications of Recombinase-aided Amplification 25

2.2.5 Advantages and Limitations of Recombinase-aided Amplification 28

2.2.6 Recent Advances of Recombinase-aided Amplification 29

2.2.7 Summary 41

2.3 Summary 41

References 42

3 Diving into the ELISA Technique: Its Role and Innovations in Public Health 45
Xin Wang, Nan Cheng, and Juewen Liu

3.1 Introduction to ELISA and Its Components 45

3.2 Recognition Molecules 47

3.2.1 Antibodies 47

3.2.2 Aptamers 49

3.2.3 Molecularly Imprinted Polymers 51

3.3 Signaling Molecules, Enzymes, and Nanozymes 51

3.3.1 Enzyme/Antibody Conjugates 52

3.3.2 Nanozymes and Nanozyme/Antibody Conjugates 53

3.3.3 Some Common Substrates in ELISA 54

3.4 Different Assay Formats 55

3.5 ELISA for Viral Detection: SARS-CoV-2 as an Example 58

3.5.1 SARS-CoV-2 Antigen Detection 58

3....