Stem Cell-Based Regenerative Medicine in Canine Practice - Bashir Gugjoo, Mudasir

Auteur(s) : Bashir Gugjoo, Mudasir

Editeur : Wiley

Langue : Anglais

Parution : 01/07/2025

Format : Moyen, de 350g à 1kg

Nombre de pages : 592.0

Résumé :

About the Author xiii

Foreword xv

Preface xvii

Abbreviations xix

About the Companion Website xxxiii

1 Canine Mesenchymal Stem Cells Sources, Properties, and Regulations for Clinical Use 1

1.1 Introduction 1

1.2 Understanding the Stem Cell 3

1.3 Sources of Dog MSCs for Proliferation 9

1.4 Why Do We Need to Culture Mesenchymal Stem Cells? 10

1.5 Why to Characterize MSCs? 11

1.6 Factors Affecting MSCs Therapeutic Outcome 12

1.7 Stem Cell Regulations in Research and Therapeutics 13

2 Osteoarthritis 27

2.1 Introduction 27

2.2 Predisposing Factors 28

2.3 OA Pathogenesis 30

2.4 MSCs and Their Potential Therapeutic Role in OA 37

2.5 In Vivo MSCs Studies 42

3 MSCs in Canine Bone Tissue Engineering 97

3.1 Introduction 97

3.2 Mesenchymal Stem Cells and Bone Regeneration 99

3.3 In Vivo Studies 107

4 Muscle, Tendon, and Ligament Affections 149

4.1 Introduction 149

4.2 Why Mesenchymal Stem Cells and Not Tendon Stem/Progenitor Cells (TSPCs)? 152

4.3 In Vivo Studies 160

4.4 Muscle Studies 175

5 Peripheral Nerve Affections 197

5.1 Introduction 197

5.2 Peripheral Nerve Injury 199

5.3 Why MSCs and Not Schwann Cells for Nerve Regeneration 206

5.4 In Vivo Peripheral Nerve Injury Studies 207

6 Central Nervous System Affections 225

6.1 Introduction 225

6.2 CNS Intrinsic Regeneration Potential 226

6.3 Exogenous Stem Cell Therapy 227

7 Myocardial and Valvular Diseases 287

7.1 Introduction 287

7.2 Why MSCs in Cardiac Diseases? 288

7.3 In Vivo MSCs Therapeutic Studies in Dogs 296

8 Oral and Esophageal Affections 341

8.1 Introduction 341

8.2 Oral Mucosal Ulcers 344

8.3 Periodontal Disease 347

8.4 Maxillary Alveolar Bone Cleft Model 359

8.5 Peri-implant Defect Models 360

8.6 Esophagus 362

9 Inflammatory Bowel Disease and Anal Furunculosis 377

9.1 Introduction 377

9.2 Why Mesenchymal Stem Cells 382

9.3 In Vivo Studies 384

10 Liver Affections 397

10.1 Introduction 397

10.2 Regenerative Medicine in Canine Hepatology 398

10.3 In Vivo Liver Regenerative Studies 404

10.4 Hepatocutaneous Syndrome 409

11 Lung Affections 419

11.1 Introduction 419

11.2 MSCs Potential Therapeutic Effects 421

11.3 In Vivo Studies 423

12 Renal Insufficiency and Urinary Bladder Affections 435

12.1 Introduction 435

12.2 In Vitro Renal Studies 437

12.3 In Vivo Renal Studies 441

12.4 Urinary Bladder 450

13 Skin Wounds and Atopic Dermatitis 461

13.1 Introduction 461

13.2 In Vivo Skin Wound-Healing Studies Using MSCs 462

13.3 Atopic Dermatitis (AD) 472

14 Diabetes 487

14.1 Introduction 487

14.2 In Vitro Studies 490

14.3 In Vivo Studies 499

15 Corneal Ulcers and Keratoconjunctivitis Sicca 517

15.1 Introduction 517

15.2 Mesenchymal Stem Cells (MSCs) Versus Corneal/Limbal Stem Cells 518

15.3 Corneal Affections 521

15.4 Keratoconjunctivitis Sicca (KCS) 527

References 532

Index 543

...

Sommaire:

About the Author xiii

Foreword xv

Preface xvii

Abbreviations xix

About the Companion Website xxxiii

1 Canine Mesenchymal Stem Cells Sources, Properties, and Regulations for Clinical Use 1

1.1 Introduction 1

1.2 Understanding the Stem Cell 3

1.2.1 MSCs Stemness or Stromalness? 4

1.2.2 MSCs Plasticity 6

1.2.3 MSCs Mobilization and Homing 7

1.2.4 Anti-inflammatory and Immunomodulatory Actions 8

1.2.5 Antimicrobial Actions 8

1.3 Sources of Dog MSCs for Proliferation 9

1.4 Why Do We Need to Culture Mesenchymal Stem Cells? 10

1.5 Why to Characterize MSCs? 11

1.6 Factors Affecting MSCs Therapeutic Outcome 12

1.7 Stem Cell Regulations in Research and Therapeutics 13

References 15

2 Osteoarthritis 27

2.1 Introduction 27

2.2 Predisposing Factors 28

2.3 OA Pathogenesis 30

2.3.1 OA Mediated Through Cartilage 30

2.3.2 OA Mediated Through Synovitis 34

2.3.3 Subchondral Bone Involvement 36

2.4 MSCs and Their Potential Therapeutic Role in OA 37

2.4.1 MSCs Chondrogenic Studies and Ex Vivo Findings 37

2.4.1.1 Culture Technique 38

2.4.1.2 Local Microenvironment 39

2.4.1.3 Source of MSCs 39

2.4.1.4 MSCs Transplantation Techniques and Drug Reactions 39

2.4.1.5 Therapeutic Properties of MSCs 40

2.5 In Vivo MSCs Studies 42

2.5.1 Preclinical Experimental Studies 42

2.5.1.1 Chondral Lesions 42

2.5.1.2 Osteochondral Defects 46

2.5.1.3 Cruciate Ligament Resection Model 51

2.5.1.4 Meniscectomy Model 54

2.5.2 Clinical Studies 55

2.5.2.1 Hip Joint OA 56

2.5.2.2 Elbow OA 61

2.5.2.3 Stifle Joint OA 66

2.5.2.4 Multiple Joint OA 66

2.5.2.5 Hip Dysplasia Induced OA 72

References 73

3 MSCs in Canine Bone Tissue Engineering 97

3.1 Introduction 97

3.2 Mesenchymal Stem Cells and Bone Regeneration 99

3.2.1 MSCs In Vitro Osteogenesis 100

3.2.1.1 Osteogenic Growth Factors/Chemicals 101

3.2.1.2 Effect of Source 103

3.2.1.3 Scaffold and MSCs Osteogenesis 105

3.2.1.4 Breed-Wise Differences 106

3.2.2 MSCs Osteogenesis 107

3.3 In Vivo Studies 107

3.3.1 Preclinical Experimental Studies in Dogs 108

3.3.1.1 Femoral Bone Defect Model 109

3.3.1.2 Radial Bone Defect Model 117

3.3.1.3 Ulnar Bone Defect Model 118

3.3.1.4 Tibial Bone Defect Model 119

3.3.1.5 Mandibular Bone Defect Models 120

3.3.2 Dog In Vivo Clinical Studies 125

3.3.2.1 Augmentation of Normal Bone Healing 128

3.3.2.2 Nonunion Radial Fracture 128

3.3.2.3 Nonunion Radial/Ulnar Fracture 129

3.3.2.4 Nonunion Femoral Fracture 130

References 131

4 Muscle, Tendon, and Ligament Affections 149

4.1 Introduction 149

4.2 Why Mesenchymal Stem Cells and Not Tendon Stem/Progenitor Cells (TSPCs)? 152

4.2.1 Tenogenic Markers 154

4.2.2 In Vitro Studies on MSCs Tenogenesis 155

4.2.2.1 Tenogenic Differentiation 155

4.2.2.2 In Vitro Tendon Healing Model 158

4.3 In Vivo Studies 160

4.3.1 Tendon Repair Models 160

4.3.1.1 Intrasynovial Second and Fifth Flexor Digitorum Profundus Model 160

4.3.1.2 Rotator Cuff Injury Model 165

4.3.1.3 Achilles Tendon Model 167

4.3.2 Clinical Tendinopathies 167

4.3.3 Ligament Repair Models 170

4.3.3.1 Partial CrCL Tear Studies 171

4.3.3.2 Complete CrCL Tear Studies 173

4.4 Muscle Studies 175

4.4.1 MSCs In Vitro Myogenic Differentiation 175

4.4.2 In Vivo Clinical Muscle Injury Studies 176