HPLC Methods for Clinical Pharmaceutical Analysis - Hermann Mascher

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Résumé :
Filling a gap in the literature for a hands-on guide focusing on everyday laboratory challenges, this English edition has been expanded and revised using the feedback received on the successful German precursor. Throughout the book, Professor Mascher draws on his 30 years of experience and provides abundant practical advice, troubleshooting and other hints highlighted in boxes, as well as a broad selection of walkthrough case studies. Based on a course taught by the author, the first part of the book intuitively explains all steps of routine bioanalysis and explains how to set up a robust, inexpensive and effi cient method for a given substance. In the second part he includes 20 worked example cases that highlight common challenges and how to overcome them. With its appendix containing tried-and-tested analytical methods for 100 clinically relevant substances from the author`s own laboratory, complete with spectral and MS data as well as literature references and basic pharmacokinetic information, this is a life-long companion for everyone working in clinical, pharmaceutical and biochemical analysis. Comments to the German book: The book comes to life through its examples, showing not only what did work in the author's laboratory, but also what didn't. ChemieReport Indispensable for novices, while even old hands will be able to expand their knowledge. A collection of analytical data for ca. 100 substances completes the book's offering, leaving almost nothing to be desired. pharmind

Biographie:
Hermann Mascher is founder and CEO of the independent contract research organization pharm-analyt based in Baden (near Vienna), Austria. A chemist by training, he has been dealing with quantitative determination of drugs, metabolites, phyto-ingredients and endogenous substances in plasma, urine and tissue for more than 30 years. Professor Mascher is regularly being consulted for analytical questions of drugs, food as well as botanical and ecological issues. He has published over 80 publications majorly in peer reviewed journals and received several awards in science and innovation for his work in the analysis of clinically important substances.

Sommaire:
Preface INTRODUCTION First Question: Determination of Ibuprofen in Plasma Second Question: Determination of Tryptophan in Urine Third Question: Determination of Paclitaxel in Tissue PLANNING OF ANALYSES Introduction Limit of Detection (LOD) and Determination (LLOQ) Detectors Structure of the Analyte Solubility of an Analyte Selection of the Detector SAMPLE PREPARATION Dilution Protein Precipitation, Overview Extraction HPLC SEPARATION HPLC Pumps Degassers Injector HPLC Columns DETECTION Detection in the Pharmaceutical/Bioanalytical Area Detection in the Clinical Area (Therapy Control/Compliance) CHEMICAL DERIVATISATION FOR DETECTION ENHANCEMENT VALIDATION CONCEPTS Realisation of the FDA Guideline PRACTICAL HINTS CONCERNING STABILITY, DESTRUCTION AND DEGRADATION PRODUCTS METABOLITES INTERNAL STANDARDS CASE STUDIES WITH INTENSIVE DISCUSSION FOR EACH SUBSTANCE Acetylcarnitine in Plasma Acetylcysteine in Plasma Acyclovir in Plasma and Urine Caffeine in Plasma Diazepam in Plasma Diclofenac in Plasma Dihydralazine in Plasma Duramycin (Moli1901) in Plasma Fluticasone Propionate in Plasma Hydroxytriamterene Sulfate and Triamterene in Plasma and Urine Ibuprofen in Plasma (also Enantiomeric Separation) Minocycline in plasma Norfloxacine in Plasma and Urine Paclitaxel in Plasma, Urine, and Tissue Paracetamol (Acetaminophen) in Plasma Pimelic Acid in Plasma and Urine 8-Prenylnaringenin in Plasma and in Different Types of Tissues Silibinin in Plasma Valnemulin in Plasma, Different Tissue Types and Animal Feed Vitamin B1 (Total Thiamine) in Plasma APPENDIX Short Description of Determination for about 100 Substances Substances listed in the Appendix A Short Explanation of Tables Presented in the Appendix

The book is clearly written and comprehensive. A lot of practical advice and tips makes it helpful for any practically oriented clinical laboratory and it can be counted as a valuable source for everyone working with chromatographic techniques in a bioanalytical laboratory. ( Analytical and Bioanalytical Chemistry , 13 November 2012) This book is more than a very successful and useful user guide, and is a valuable tool for the laboratorywork, not only for clinical analysts, but also for biochemists, pharmacists, etc. In addition to a compact targeted representation of the most important theoretical foundations for the planning and execution of (clinical) analysis (sample preparation, HPLC separation, usage of different modes, detection capabilities, derivatization techniques to the point of validation), the readers receive valuable information for their work, which are explained on the basis of practical examples [pages 1--64]. ( ChemMedChem , 1 November 2012)