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Présentation Biosensors Format Relié
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Résumé : About the Authors ix Preface xiii Acknowledgments xvii List of Abbreviations xix About the Companion Website xxi 1 Fundamentals of Biomedical Sensors 1 1.1 Introduction 1 1.2 Classification of Biosensors 5 1.3 Elements of Biosensors 5 1.4 Bio-recognition Elements 5 1.4.1 Nucleic Acids 5 1.4.2 Aptamers 7 1.4.3 Enzymes 7 1.4.4 Antibodies 7 1.4.5 Bacteriophages 9 1.4.6 MIPs 10 1.4.7 Biosensors Based on Whole Cells 12 1.5 Sensing Techniques 14 1.5.1 ECL Biosensors 14 1.5.2 Optical Biosensors 15 1.5.2.1 Plasmonic Biosensors 15 1.5.2.2 Interferometric Sensors 18 1.5.2.3 Colorimetric Sensors 19 1.5.2.4 SERS-Based Biosensors 21 1.5.2.5 Fluorescence-Based Biosensors 23 1.5.2.6 Photonic Crystal-Fiber-Based Biosensors 24 1.5.3 Mass Biosensors 25 References 27 2.1 Introduction 39 2.2 Requirements of Immobilization 41 2.3 Immobilization Methods 42 2.3.1 Adsorption Immobilization or Electrostatic Interaction Method 42 2.3.2 Entrapment 43 2.3.3 Membrane Encapsulation 43 2.3.4 Covalent Immobilization 45 2.4 Materials Used in Different Immobilization Techniques and Supports 47 2.5 Future Prospects and Possibilities in Immobilization Technology 50 2.6 Summary 53 References 55 3 Nucleic Acid-Based Biosensors 63 3.1 Introduction 63 3.2 ECL Biosensor Designs 64 3.3 Nucleic Acid-Based Colorimetric Biosensors 67 3.4 Nucleic Acid-Based Fluorescent Biosensor 70 3.5 Nucleic Acid-Based Plasmonic Biosensor 71 3.6 SERS-Based Nucleic Acid Biosensors 76 3.7 Interferometric NA Biosensors 78 3.8 Conclusions and Future Prospects 81 References 81 4 Antibody-Based Biosensors 91 4.1 Polyclonal Antibody and Monoclonal Antibody 91 4.2 Cardiovascular Biomarker, Autoimmune Disorder, and Cholesterol Biomarker 94 4.2.1 Cancer Detection 94 4.2.2 Tuberculosis Detection 98 4.2.3 Cardiovascular Disease 104 4.2.4 Autoimmune Disease 105 4.2.5 Detection of Stress Biomarkers 105 4.2.6 Cholesterol Disease 107 4.2.7 Glucose Measurement 110 4.2.8 Microbe Detection: Viruses, Bacteria, Archaea, Fungi, and Protists 112 4.2.9 Toxins Detection 112 4.3 Conclusions and Future Directions 118 References 118 5 Aptamer-Based Biosensors 139 5.1 Introduction 139 5.2 Selection of Aptamers and Design Strategies 142 5.2.1 Modified Bases 143 5.2.2 Structural Modeling 143 5.2.3 Cell-SELEX 145 5.2.4 Counter-SELEX 145 5.2.5 High-throughput Sequencing 147 5.2.6 In Silico SELEX 147 5.2.7 Structure-Switching Signaling-Based Aptamers 148 5.2.8 Folding Aptamer-Based Biosensors 150 5.2.9 Split Aptamers 153 5.3 Sepsis, Pathogens, and Biomarkers to Detect Disease and Pollutants 155 5.4 Small Biomolecules 158 5.5 Summary 163 References 163 6 Bacteriophage-Based Biosensors 177 6.1 Introduction 177 6.1.1 Reporter Bacteriophages 180 6.1.2 Stained Phages 180 6.1.3 Lytic Phages 181 6.1.4 Capturing Phages 181 6.1.5 Phage Receptor-Binding Proteins 181 6.2 Design of Bacteriophage-Based Biosensor 182 6.3 Future Prospects 195 6.4 Summary 196 References 196 7 Peptide-Based Biosensors 203 7.1 Introduction 203 7.2 Synthesis Methods 204 7.2.1 Design of ECL Biosensors 205 7.2.2 ...
2 Immobilization Techniques for Bioreceptors 39
Biographie:
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Sommaire: About the Authors ix Preface xiii Acknowledgments xvii List of Abbreviations xix About the Companion Website xxi 1 Fundamentals of Biomedical Sensors 1 1.1 Introduction 1 1.2 Classification of Biosensors 5 1.3 Elements of Biosensors 5 1.4 Bio-recognition Elements 5 1.4.1 Nucleic Acids 5 1.4.2 Aptamers 7 1.4.3 Enzymes 7 1.4.4 Antibodies 7 1.4.5 Bacteriophages 9 1.4.6 MIPs 10 1.4.7 Biosensors Based on Whole Cells 12 1.5 Sensing Techniques 14 1.5.1 ECL Biosensors 14 1.5.2 Optical Biosensors 15 1.5.2.1 Plasmonic Biosensors 15 1.5.2.2 Interferometric Sensors 18 1.5.2.3 Colorimetric Sensors 19 1.5.2.4 SERS-Based Biosensors 21 1.5.2.5 Fluorescence-Based Biosensors 23 1.5.2.6 Photonic Crystal-Fiber-Based Biosensors 24 1.5.3 Mass Biosensors 25 References 27 2 Immobilization Techniques for Bioreceptors 39 2.1 Introduction 39 2.2 Requirements of Immobilization 41 2.3 Immobilization Methods 42 2.3.1 Adsorption Immobilization or Electrostatic Interaction Method 42 2.3.2 Entrapment 43 2.3.3 Membrane Encapsulation 43 2.3.4 Covalent Immobilization 45 2.4 Materials Used in Different Immobilization Techniques and Supports 47 2.5 Future Prospects and Possibilities in Immobilization Technology 50 2.6 Summary 53 References 55 3 Nucleic Acid-Based Biosensors 63 3.1 Introduction 63 3.2 ECL Biosensor Designs 64 3.3 Nucleic Acid-Based Colorimetric Biosensors 67 3.4 Nucleic Acid-Based Fluorescent Biosensor 70 3.5 Nucleic Acid-Based Plasmonic Biosensor 71 3.6 SERS-Based Nucleic Acid Biosensors 76 3.7 Interferometric NA Biosensors 78 3.8 Conclusions and Future Prospects 81 References 81 4 Antibody-Based Biosensors 91 4.1 Polyclonal Antibody and Monoclonal Antibody 91 4.2 Cardiovascular Biomarker, Autoimmune Disorder, and Cholesterol Biomarker 94 4.2.1 Cancer Detection 94 4.2.2 Tuberculosis Detection 98 4.2.3 Cardiovascular Disease 104 4.2.4 Autoimmune Disease 105 4.2.5 Detection of Stress Biomarkers 105 4.2.6 Cholesterol Disease 107 4.2.7 Glucose Measurement 110 4.2.8 Microbe Detection: Viruses, Bacteria, Archaea, Fungi, and Protists 112 4.2.9 Toxins Detection 112 4.3 Conclusions and Future Directions 118 References 118 5 Aptamer-Based Biosensors 139 5.1 Introduction 139 5.2 Selection of Aptamers and Design Strategies 142 5.2.1 Modified Bases 143 5.2.2 Structural Modeling 143 5.2.3 Cell-SELEX 145 5.2.4 Counter-SELEX 145 5.2.5 High-throughput Sequencing 147 5.2.6 In Silico SELEX 147 5.2.7 Structure-Switching Signaling-Based Aptamers 148 5.2.8 Folding Aptamer-Based Biosensors 150 5.2.9 Split Aptamers 153 5.3 Sepsis, Pathogens, and Biomarkers to Detect Disease and Pollutants 155 5.4 Small Biomolecules 158 5.5 Summary 163 References 163 6 Bacteriophage-Based Biosensors 177 6.1 Introduction 177 6.1.1 Reporter Bacteriophages 180 6.1.2 Stained Phages 180 6.1.3 Lytic Phages 181 6.1.4 Capturing Phages 181 6.1.5 Phage Receptor-Binding Proteins 181 6.2 Design of Bacteriophage-Based Biosensor 182 6.3 Future Prospects 195 6.4 Summary 196 References 196 7 Peptide-Based Biosensors 203 7.1 Introduction 203 7.2 Synthesis Methods 204 7.2.1 Design of ECL Biosensors 205 7.2....
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