Introduction to Biological Imaging - Auer, Manfred

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Résumé :
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Biographie:
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Sommaire:
1. Introduction: A brief history of imaging 2. Basics of Atomic and Molecular Physics 2.1. Matter and energy 2.2. Electromagnetic spectrum 2.3. Interaction of radiation with matter 3. Basics of Optics 3.1. Properties of light 3.2. Reflection 3.3. Refraction 3.4. Dispersion 3.5. Lenses 3.6. Numerical aperture 3.7. Scattering 3.8. Interference 3.9. Diffraction 3.10. Airy disk 3.11. Point spread function 3.12. Resolution 3.13. Aberrations 3.14. Polarization 3.15. Photoelectric effect 3.16. Valence electron transitions 3.17. Vibrational and rotational transitions 3.18. Stimulated emission 4. Basics of Fourier Math 5. Basics of Imaging and Image Formation 5.1. Goals of imaging 5.2. Source of radiation 5.3. Beam collimation 5.4. Optical lenses 5.5. Lens-free imaging/near-field 5.6. Signal detection and detectors 5.7. Modulation-, contrast- and optical-transfer functions 5.8. Field of view, coverage 5.9. Flood-beam versus spot-scan imaging 5.10. Radiation damage 5.11. Spectroscopic Imaging 5.12. Multi-dimensional and multi-channel imaging 5.13. Sample preparation requirements 5.14. Labels and labeling 5.15. Label-free imaging 5.16. 2D versus 3D imaging 5.17. Structural versus functional imaging 5.18. Correlative imaging 6. Basics of Image Processing and Image Analysis 6.1. Digital Images 6.2. Image restoration, enhancement and reconstruction 6.3. Visualization 6.4. Image segmentation 6.5. Measurements/quantitative analysis 7. Techniques for Macromolecular Structure Determination 7.1. X-ray Crystallography 7.2. Cryo-Electron Microscopy 7.3. NMR spectroscopy 7.4. Small angle scattering: SAXS & SANS 7.5. Atomic Force Microscopy (AFM) 8. Techniques for Cellular Ultrastructure: Architectural Imaging 8.1. Overview 8.2. Historical Perspective/Milestones 8.3. Theoretical Background 8.4. Sample Preparation 8.5. Instrumentation 8.6. Data Collection and 3D Reconstruction 8.7. Data Visualization and Analysis 9. Techniques for Cells and Tissues: Characterization and Localization Imaging 9.1. Overview 9.2. Historical Perspective/Milestones 9.3. Theoretical Background 9.4. Instrumentation/Experimental Setup 9.5. Data Collection 9.6. Data Analysis 9.7. Visualization 10. Techniques for Cell and Tissue Chemical Imaging 10.1. X-ray Microanalysis 10.2. Raman Imaging 10.3. FTIR 10.4. Mass Spectrometry 11. Techniques for Tissue and Organ Medical Imaging 11.1. Positron Emission Tomography (PET) 11.2. Computed (Axial) Tomography 11.3. Magnetic Resonance Imaging (MRI) 11.4. Medical Ultrasound 11.5. Bioluminescence 11.6. Optical Coherence Tomography (OCT) 11.7. Histology/Histopathology 12. Future of Bioimaging 12.1. Future of Macromolecular Imaging 12.2. Future of (Sub)Cellular and Tissue Imaging 12.3. Future of Chemical Imaging 12.4. Future of Medical Imaging 12.5. Future of Image Processing and Image Analysis 12.6. Future of Multiscale Imaging 12.7. Future of Multimodal Imaging - Correlative Imaging 12.8. Future of Information Integration - Multiscale, Multimodal and Integrated