PCR Protocols -

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Sommaire:
Part I. Introduction to PCR A Short History of the Polymerase Chain Reaction John M. S. Bartlett and David Stirling PCR Patent Issues Peter Carroll and David Casimir Equipping and Establishing a PCR Laboratory Susan McDonagh Quality Control in PCR David Stirling Part II. Preparation of Nucleic Acid Templates Extraction of Nucleic Acid Templates John M. S. Bartlett Extraction of DNA from Whole Blood John M. S. Bartlett and Anne White DNA Extraction from Tissue Helen Pearson and David Stirling Extraction of DNA from Microdissected Archival Tissues James J. Going RNA Extraction from Blood Helen Pearson RNA Extraction from Frozen Tissue RNA Extraction from Tissue Sections Dual DNA/RNA Extraction David Stirling and John M. S. Bartlett DNA Extraction from Fungi, Yeast, and Bacteria Isolation of RNA Viruses from Biological Materials Extraction of Ancient DNA Wera M. Schmerer DNA Extraction from Plasma and Serum Technical Notes for the Detection of Nucleic Acids Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels Part III. Basic PCR Methods PCR Primer Design David L. Hyndman and Masato Mitsuhashi Optimization of Polymerase Chain Reactions Haiying Grunenwald Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content: Amplification of the Intron 22 Inversion of the FVIII Gene Rapid Amplification of cDNA Ends Xin Wang and W. Scott Young III Randomly Amplified Polymorphic DNA Fingerprinting: The Basics Ranil S. Dassanayake and Lakshman P. Samaranayake Microsphere-Based Single Nucleotide Polymorphism Genotyping Marie A. Iannone, J. David Taylor, Jingwen Chen, May-Sung Li, Fei Ye, and Michael P. Weiner Ligase Chain Reaction William H. Benjamin, Jr., Kim R. Smith, and Ken B. Waites Nested RT-PCR in a Single Closed Tube Antonio Olmos, Olga Esteban, Edson Bertolini, and Mariano Cambra Direct PCR from Serum: Application to Viral Genome Detection Kenji Abe Long PCR Amplification of Large Fragments of Viral Genomes: A Technical Overview Raymond Tellier, Jens Bukh, Suzanne U. Emerson, and Robert H. Purcell Long PCR Methodology Part IV. Ultrasensitive and Quantitative PCR Qualitative and Quantitative PCR: A Technical Overview Ultrasensitive PCR Detection of Tumor Cells in Myeloma Friedrich W. Cremer and Marion Moos Ultrasensitive Quantitative PCR to Detect RNA Viruses Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics Quantitation of Multiple RNA Species Ron Kerr Part V. Transcriptome Analysis Differential Display: A Technical Overview AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs Orlando Dominguez, Lidia Sabater, Yaqoub Ashhab, Eva Belloso, and Ricardo Pujol-Borrell PCR Fluorescence Differential Display Kostya Khalturin, Sergej Kuznetsov, and Thomas C. G. Bosch Microarray Analysis Using RNA Arbitrarily Primed PCR Steven Ringquist, Gaelle Rondeau, Rosa-Ana Risques, Takuya Higashiyama, Yi-Peng Wang, Steffen Porwollik, David Boyle, Michael McClelland, and John Welsh Oligonucleoti