Flow Cytometry - Alice Longobardi Givan

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Résumé :

A VALUABLE RESOURCE FOR INTRODUCING FLOW CYTOMETRY, PROVIDING A FOUNDATION FOR UNDERSTANDING ITS IMPORTANCE IN VARIOUS FIELDS

The newly updated and revised Flow Cytometry: First Principles, Third Edition covers flow cytometric theory and the fundamentals of instrumentation and data analysis using examples from a wide range of scientific and clinical applications. Readers will find and learn about both standard and advanced flow cytometric instrumentation, methods for analyzing flow data, and applications of the technique ranging from immunology, clinical diagnoses, DNA analysis, and cell sorting to cell function, plant biology, and molecular biology. Each chapter starts with basic knowledge and gradually expands on the subject matter, enabling readers to assess the ways that flow cytometry has been used in their respective fields and the ways that it might be applied in the future. A selected number of annotated references are given, aiming to point readers into relevant literature. There is an extensive glossary at the end of the book.

Written by two experts and highly illustrated, Flow Cytometry: First Principles, Third Edition includes information on:

• How flow cytometry is used to analyze cells
• How a flow cytometer can be used to separate different types of cells from each other
• Fluidics, electronics, and optics (including lasers, fluorochromes, and filters)
• Clinical applications of flow cytometry

An indispensable authoritative text on flow cytometry, the third edition of Flow Cytometry: First Principles is an essential resource for scientists, clinicians, technologists, instructors, and students....

Biographie:

1. The Past as Prologue
2. Setting the Scene
3. Instrumentation: Into the Black Box
4. Information: Harnessing the Data
5. Seeing the Light: Lasers, Fluorochromes, Mirrors, Prisms, and Filters
6. Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating
7. Cells from Within: Intracellular Proteins
8. Cells from Within: DNA in Life and Death
9. Assays of Function
10. The Sorting of Cells
11. Disease and Diagnosis: The Clinical Laboratory
12. Out of the Mainstream: Biological Frontiers
13. Out of the Mainstream Technological Frontiers
14. Flowing On: The Future