Methods in Neurobiology - Lahue, Robert

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Sommaire:
1 Behavioral Techniques in Pharmacological and Neuropharmacological Analysis.- 1. Introduction.- 2. Basic Principles.- 3. Locomotor Excitation and Inhibition.- 4. Habituation.- 5. Appetitively Motivated Operant Behaviors.- 6. Aversively Motivated Behaviors.- 7. Overview.- References.- 2 Behavioral and Biochemical Methods to Study Brain Responses to Environment and Experience.- 1. Introduction.- 2. Environmental and Training Techniques.- 3. Behavioral Techniques to Test Effects of Prior Experience.- 4. Biochemical Techniques.- References.- 3 Cell Fractionation.- 1. Introduction.- 2. Basic Information on Centrifugation.- 3. Centrifuges.- 4. Homogenization.- 5. Differential Centrifugation.- 6. Analytical vs. Preparative Strategies.- References.- Selected Bibliography of Fractionation Literature.- 4 Polyacrylamide Gel Electrophoresis: Principles, Techniques, and Micromethods.- 1. Introduction.- 2. Basic Concepts.- 3. Outline of Electrophoretic Techniques.- 4. Practical Aspects of Electrophoresis and Micromethods.- 5. Related Techniques and Analysis of Gel Patterns.- 6. Interfacing Electrophoresis with Other Techniques.- 7. Concluding Remarks.- References.- 5 Classic Methods in Neuroanatomy.- 1. Introduction.- 2. Dissection and Gross Anatomy of the Brain.- 3. Reconstruction Methods.- 4. Fixation and General Histological Procedure.- 5. Nissi Stains.- 6. Myelin Stains.- 7. Reduced-Silver Methods.- 8. Golgi Method.- 9. Histological Methods.- References.- 6 Fluorescence Microscopy of Biogenic Monoamines.- 1. Introduction.- 2. Fluorescence Microscopy and Microspectrofluorometry.- 3. Visualization of Biogenic Monoamines.- 4. Fluorescence-Histochemical Techniques for Identification and Differentiation of Biogenic Monoamines.- 5. General Conclusion.- References.- 7 Electron Microscopy in Neurobiology.- 1. Introduction.- 2. The Transmission Electron Microscope: Basic Principles and Design.- 3. Electron-Microscopic Histology.- 4. Applications of Electron Microscopy in Neurobiology.- 5. Quantitative Stereology in Electron Microscopy.- References.- 8 Scanning Electron Microscopy: Applications to Neurobiology.- 1. Fundamentals of Scanning Electron Microscopy.- 2. Specific Applications of Scanning Electron Microscopy to Neurobiology.- References.- 9 Autoradiography in the Nervous System.- 1. Introduction.- 2. Application of Autoradiography in Different Fields of Neurobiological Research.- 3. Basic Principles of Autoradiography.- 4. Methods.- 5. Critical Evaluation of Anterograde Tracing Techniques.- References.- 10 Isotope Methods.- 1. Introduction.- 2. Physical Background.- 3. Isotopes of Neurobiological Interest.- 4. Labeled Compounds.- 5. Measurement of Isotopes.- 6. Statistics of Radioactivity Detection.- 7. Data Analysis in Neurobiological Applications.- References.