Molecular Imaging - Khalid Shah

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Résumé :
In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology? series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.

Sommaire:
Bioluminescence resonance energy transfer (BRET) Imaging in Plant Seedlings and Mammalian Cells.- Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and Mice.- Hybrid Raman-Fluorescence Microscopy on Single Cells using Quantum Dots.- Labeling of Mesenchymal Stem Cells with Bioconjugated Quatum Dots.- Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence.- Imaging Fate of Stem Cells at a Cellular Resolution in the Brains of Mice.- Magnetic Resonance Imaging of Brain Inflamation using Microparticles of Iron Oxide.- Optical Characterization of Arterial Apoptosis.- Intravital Near-Infrared Fluorescence Molecular Imaging of Atherosclerosis.- MR Imaging of Transplanted Stem Cells in Myocardial Infarction.- In Vivo Imaging of the Dynamics of Different Variants of EGFR in Glioblastomas.- Fluorescence lifetime-based optical molecular imaging.- PET maging of avb3 Integrins in Cancer Patients.- Quantitative Approaches to Amyloid Imaging.- Molecular imaging of myocardial remodeling after infarction.- Dual-radionuclide Brain SPECT for the Differential Diagnosis of Parkinsonism.

From the reviews:

The book covers many aspects of molecular imaging at the cellular and subcellular level and within intact living organisms. The techniques are well described and protocols are provided in detail, with a lot of technical specifications, also concerning instrumentation settings and the software used. All this information is of fundamental importance for imaging measurements and is seldom reported in the scientific literature. .... can be recommended to students, research fellows, and technicians working or planning to work with molecular imaging techniques. (Elisa Michelini, Analytical and Bioanalytical Chemistry, Vol. 403, 2012)