Bacterial Pathogenesis -

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Résumé :
Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria.- Formation and Analysis of Mono-Species and Polymicrobial Oral Biofilms in Flow-Cell Models.- Isolation and Purification of Mycobacterial Extracellular Vesicles (EVs).- Strategies to Isolate Extracellular Vesicles from Gram - and Gram + Bacteria.- Total Bacterial RNA Isolation and Northern Blotting Analysis.- Phylogenetic Analysis of Bacterial Pathogen Genomes.- Determination of Growth Rate and Virulence Plasmid Copy Number during Yersinia pseudotuberculosis Infection Using Droplet Digital PCR.- Methods to Analyze the Contribution of Complement Evasion Factor (CEF) to Streptococcus pyogenes Virulence.- Expression of the Bacterial Enzyme IdeS Using a GFP Fusion in the Yeast Saccharomyces cerevisiae.- Mass Spectrometry-Based Methods to Determine the Substrate Specificities and Kinetics of N-Linked Glycan Hydrolysis by Endo-?-N-Acetylglucosaminidases.- Identification of Substrates of Secreted Bacterial Protease by APEX2-Based Proximity Labeling.- Affinity-Purification Combined with Crosslinking Mass Spectrometry for Identification and Structural Modeling of Host-Pathogen Protein-Protein Complexes.- Elucidating the Stoichiometries of Host-Pathogen Protein Interactions with Mass Photometry.- Analysis of Neutrophil and Monocyte Inflammation Markers in Response to Gram-Positive Anaerobic Cocci.- Quantification of Phagocytosis Using Flow Cytometry.- Anti-Bacterial Neutrophil Effector Response: Ex Vivo Quantification of Regulated Cell Death Associated with Extracellular Trap Release.- Measurement of Antibody Binding Affinity on Bacterial Surfaces Using Flow Cytometry.- Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus.- In Vivo Profiling of the Vascular Cell Surface Proteome in Murine Models of Bacteremia.- Barcoded Consortium Infections: A Scalable, Internally Controlled Method to Study Host Cell Binding and Invasion by Pathogenic Bacteria.- A Murine Mycobacterium marinum Infection Model for Longitudinal Analyses of Disease Development and the Inflammatory Response.- In Vitro Approaches for the Study of Pneumococcal-Neuronal Interaction and Pathogenesis....

Sommaire:
This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.