Biochemistry - Donald Voet

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Résumé :
The Gold Standard in Biochemistry text books. Biochemistry 4e, is a modern classic that has been thoroughly revised. Don and Judy Voet explain biochemical concepts while offering a unified presentation of life and its variation through evolution. It incorporates both classical and current research to illustrate the historical source of much of our biochemical knowledge....

Biographie:

Guide to Media Resources xvi

Part I Introduction and Background 1

Chapter 1 Life 3

1. Prokaryotes 3

2. Eukaryotes 6

3. Biochemistry: A Prologue 14

4. Genetics: A Review 19

5. The Origin of Life 28

6. The Biochemical Literature 34

Chapter 2 Aqueous Solutions 40

1. Properties of Water 40

2. Acids, Bases, and Buffers 45

Chapter 3 Thermodynamic Principles: A Review 52

1. First Law of Thermodynamics: Energy Is Conserved 52

2. Second Law of Thermodynamics: The Universe Tends Toward Maximum Disorder 54

3. Free Energy: The Indicator of Spontaneity 57

4. Chemical Equilibria 58

Appendix: Concentration Dependence of Free Energy 61

Part II Biomolecules 65

Chapter 4 Amino Acids 67

1. The Amino Acids of Proteins 67

2. Optical Activity 73

3. Nonstandard Amino Acids 78

Chapter 5 Nucleic Acids, Gene Expression, and Recombinant DNA Technology 82

1. Nucleotides and Nucleic Acids 82

2. DNA Is the Carrier of Genetic Information 85

3. Double Helical DNA 88

4. Gene Expression and Replication: An Overview 95

5. Molecular Cloning 104

Chapter 6 Techniques of Protein and Nucleic Acid Purifications 129

1. Protein Isolation 129

2. Solubilities of Proteins 133

3. Chromatographic Separations 135

4. Electrophoresis 146

5. Ultracentrifugation 152

6. Nucleic Acid Fractionation 156

Chapter 7 Covalent Structures of Proteins and Nucleic Acids 163

1. Primary Structure Determination of Proteins 164

2. Nucleic Acid Sequencing 176

3. Chemical Evolution 185

4. Bioinformatics: An Introduction 194

5. Chemical Synthesis of Polypeptides 205

6. Chemical Synthesis of Oligonucleotides 209

Chapter 8 Three-Dimensional Structures of Proteins 221

1. Secondary Structure 221

2. Fibrous Proteins 232

3. Globular Proteins 241

4. Protein Stability 259

5. Quaternary Structure 266

Appendix: Viewing Stereo Pictures 271

Chapter 9 Protein Folding, Dynamics, and Structural Evolution 278

1. Protein Folding: Theory and Experiment 278

2. Folding Accessory Proteins 290

3. Protein Structure Prediction and Design 302

4. Protein Dynamics 306

5. Conformational Diseases: Amyloids and Prions 309

6. Structural Evolution 316

Chapter 10 Hemoglobin: Protein Function in Microcosm 323

1. Hemoglobin and Myoglobin Function 323

2. Structure and Mechanism 331

3. Abnormal Hemoglobins 341

4. Allosteric Regulation 347

APPENDIX: Derivation of Symmetry Model Equations 354

Chapter 11 Sugars and Polysaccharides 359

1. Monosaccharides 359

2. Polysaccharides 365

3. Glycoproteins 373

Chapter 12 Lipids and Membranes 386

1. Lipid Classification 386

2. Properties of Lipid Aggregates 393

3. Biological Membranes 399

4. Membrane Assembly and Protein Targeting 418

5. Lipoproteins 449

Part III Mechanisms of Enzyme Action 467

Chapter 13 Introduction to Enzymes 469

1. Historical Perspective 469

2. Substrate Specificity 470

3. Coenzymes 473

4. Regulation of Enzymatic Activity 474

5. A Primer of Enzyme Nomenclature 479

Chapter 14 Rates of Enzymatic Reactions 482

1. Chemical Kinetics 482

2. Enzyme Kinetics 487

3. Inhibition 492

4. Effects of pH 496

5. Bisubstrate Reactions 497

Appendix: Derivations of Michaelis-Menten Equation...

Sommaire:
Guide to Media Resources. PART I INTRODUCTION AND BACKGROUND. 1. Life. 2. Aqueous Solutions. 3. Thermodynamic Principles: A Review. PART II: BIOMOLECULES. 4. Amino Acids. 5. Nucleic Acids, Gene Expression, and Recombinant DNA Technology. 6. Techniques of Protein and Nucleic Acid Purification. 7. Covalent Structures of Proteins and Nucleic Acids. 8. Three-Dimensional Structures of Proteins. 9. Protein Folding, Dynamics, and Structural Evolution. 10. Hemoglobin: Protein Function in Microcosm. 11. Sugars and Polysaccharides. 12. Lipids and Membranes. PART III: MECHANISMS OF ENZYME ACTION. 13. Introduction to Enzymes. 14. Rates of Enzymatic Reactions. 15. Enzymatic Catalysis. PART IV: METABOLISM. 16. Introduction to Metabolism. 17. Glycolysis. 18. Glycogen Metabolism. 19. Signal Transduction. 20. Transport Through Membranes. 21. Citric Acid Cycle. 22. Electron Transport and Oxidative Phosphorylation. 23. Other Pathways of Carbohydrate Metabolism. 24. Photosynthesis. 25. Lipid Metabolism. 26. Amino Acid Metabolism. 27. Energy Metabolism: Integration and Organ Specialization. 28. Nucleotide Metabolism. PART V: EXPRESSION AND TRANSMISSION OF GENETIC INFORMATION. 29. Nucleic Acid Structures. 30. DNA Replication, Repair, and Recombination. 31. Transcription. 32. Translation. 33. Viruses: Paradigms for Cellular Function. 34. Eukaryotic Gene Expression. 35. Molecular Physiology. (Chapters 33-35 are available on our website, www.wiley.com/college/voet)....